WebIts tunable and relatively slow kinetics make Opto-CRAC most suitable for generating customized Ca 2+ oscillation patterns in non-excitable cell types, such as cells of the immune and hematopoietic systems. WebJan 12, 2024 · We use this "optoDroplet" system to study condensed phases driven by the IDRs of various RNP body proteins, including FUS, DDX4, and HNRNPA1. Above a concentration threshold, these constructs undergo light-activated phase separation, forming spatiotemporally definable liquid optoDroplets.

Frontiers Engineering Light-Control in Biology

WebOptogenetic Control of Gene Expression Using Cryptochrome 2 and a Light-Activated Degron. Methods Mol Biol, 2024 βH-spectrin is required for ratcheting apical pulsatile constrictions during tissue invagination. EMBO Rep, 2024 Early But Not Delayed Optogenetic RAF Activation Promotes Astrocytogenesis in Mouse Neural Progenitors. J Mol Biol, 2024 WebChannelrhodopsin-2 (ChR2), the first widely adopted optogenetic tool, is also a blue light activated cation channel. ChR2 is preferred over ChR1 because ChR2 has higher conductance at physiological pH and trafficks well to the membrane. Excitatory (Depolarizing) ChR Variants. chloe duckworth instagram

Addgene: Myristoylated optoDroplet

WebThe Dual Characteristics of Light-Induced Cryptochrome 2, Homo-oligomerization and Heterodimerization, for Optogenetic Manipulation in Mammalian Cells. ACS Synth Biol, … WebOne way to achieve this light-mediated change in protein valency is to use the optogenetic protein Cry2, the photolyase homology region of a cryptochrome from Arabidopsis thaliana, that undergoes light-sensitive self-association upon blue … WebCRY2 is a FAD containing photoreceptor from Arabidopsis.Arabidopsis cryptochrome 2 (CRY2) can simultaneously carry out light dependent CRY2-CRY2 homooligomerization and CRY2-CIB1 heterodimerization, both of which have been widely used to optically control intracellular processes.Both configurations can be activated by blue light. Solutions and … grass shoots