Nettet1. Trio breeding groups are best suited for the propagation of inbred, transgenic, or other strains of mice which generate small numbers of pups or are difficult to breed. Crosses … Nettet1. Trio breeding groups are best suited for the propagation of inbred, transgenic, or other strains of mice which generate small numbers of pups or are difficult to breed. Crosses that produce larger litters are best propagated by a pair (defined as one male and one female) breeding strategy. 2.

Breeding Strategies for Genetically Modified Mice - PubMed

NettetBreeding Strategies for Maintaining Colonies of Laboratory Mice A Jackson Laboratory Resource Manual Th is manual describes breeding strategies and techniques for … NettetThe first and most critically important step in designing ANY breeding scheme is to determine what genotype(s) of mice you need for both your experimental AND your control mice. Whether you are dealing … hawthorne projection

Breeding strategies for tissue-specific knockout mice

NettetIn general, mouse pups should be weaned from their mother between 21-26 days of age. At this time, male and female pups must be separated into same-sex groups to avoid accidental breeding when mice reach puberty at 6-8 weeks of age. If litters are not weaned promptly, the cages will become overcrowded. NettetBecause cre transgenes generally don’t need to be homozygous to efficiently recombine loxP-flanked (floxed) alleles, many investigators never bother to breed their cre … NettetThis chapter covers the design and construction of loxP flanked alleles and refers to the vectors, ES cells, and mice generated by the European conditional mouse mutagenesis (EUCOMM) project. We further describe the design and use of Cre and CreER(T2) transgenic mice and a convenient breeding strategy to raise conditional mutants and … hawthorne primary care - hawthorne