WebJan 1, 1979 · This chapter discusses the preparation of Sendai virus for cell fusion. Positive allantoic fluids are pooled aseptically and clarified of urates and cell debris by centrifuging. ... The inactivated virus is diluted to 4000 HAU/ml with Earle's BSS (2 parts virus + 3 parts BSS), ampuled in 1.1-ml lots, snap-frozen in a mixture of solid CO 2 and ... WebThe close apposition of infected and uninfected cells occurring during the agglutination pro- duced by treatment with ultraviolet-inactivated Sendai virus in the cold, rather than complete cell ...

ERK-mediated autophagy promotes inactivated Sendai virus (HVJ …

WebPreparation of Sendai Virus for Cell Fusion. Reet Tees, in Immunological Methods, 1979. V ASSAY OF INFECTIVITY. The inactivated virus is diluted in tenfold steps (10 −1 to 10 −6) in Earle's BSS and 0.05 ml of each dilution is inoculated allantoically into a group of three eggs (see Section I). After incubation at 35°C for 68–72 hr, about ... HVJ-E was manufactured at GenomIdea Inc. (Osaka, Japan). HVJ was irradiated by the treatment with β-propiolactone and ultraviolet irradiation, which caused the alkylation and the fragmentation of the RNA genome. HVJ-E was purified by four steps of column chromatography, stabilized by lyophilization and … See more This study was a single-arm, open-label, single-center, phase I/II study of HVJ-E in patients with CRPC who were docetaxel resistant or could not receive docetaxel treatments. The primary end points were to evaluate safety and … See more Eligible patients had to have CRPC and be docetaxel resistant or unable to receive docetaxel treatment and have a prostate in which HVJ-E could be administered. Patients were … See more Patients were monitored in hospital from day 1 to day 14 and at each outpatient visit on days 21 and 28 of each cycle. The National Cancer Institute’s Common Terminology Criteria for Adverse Events version 4.0 was used … See more A 3+3 design was used to determine the maximum tolerated dose of HVJ-E administration. HVJ-E was administered directly to the … See more gwyneth paltrow weight loss

Interferon inhibits Sendai virus-induced cell fusion: an effect ... - PNAS

WebJul 18, 2016 · Some studies [37–38] have found that UV-inactivated Sendai virus is different from a saline control, which is unable to replicate in cells because the genome is destroyed by UV irradiation, but still contains the complete structure of the live HVJ envelope containing F, HN, and M proteins. These proteins have displayed antitumor effect ... WebSinovac Inactivated Inactivated 2 0, 14 days IM NCT04383574 NCT04352608 NCT04456595 669/UN6.KEP/EC/2024 ... Inactivated Inactivated whole virus National Research Centre, Egypt SARS-CoV2 Pre-Clinical ... Non-Replicating Viral Vector Sendai virus vector ID Pharma SARS-CoV2 Pre-Clinical gwyneth paltrow website goop