NettetHoechst33342 is cell permeable and usually used in live cell imaging. However for long term imaging, the UV laser illumination creates a lot of photo-toxicity. Its … NettetIncubate cells in the diluted antibody in 1% BSA in PBST in a humidified chamber for 1 h at room temperature or overnight at 4°C. Decant the solution and wash the cells three times in PBS, 5 min each wash. Incubate cells with the secondary antibody in 1% BSA for 1 h at room temperature in the dark. Decant the secondary antibody solution and ...
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NettetHoechst 33258 Staining Dye Solution (ab228550) is a fluorescent stain for labeling DNA in fluorescence microscopy. This product may be used in fluorescence microscopy, … NettetThe optimal concentration for primary antibodies can vary widely; concentrations for initial testing usually start around 1 ug/mL or higher. Secondary antibodies are typically used at 1 ug/mL for cell staining and as low as ≥50 ng/mL for near-infrared western detection. Back to Top View All Products methodist church winner sd
Cell Cycle using Hoechst 33342 in Unfixed Cells - Rutgers …
NettetVolume of Water needed to reconstitute specific mass of Hoechst 33342 *20 mM solution in water* to given concentration. Note that volume is only for preparing stock solution. … NettetHoechst 33342 (2'- [4-ethoxyphenyl]-5- [4-methyl-1-piperazinyl]-2,5'-bi-1H-benzimidazole trihydrochloride trihydrate) is a cell-permeable DNA stain that is excited by ultraviolet … NettetHoechst 33342 Catalog No.S0485 Synonyms: Pibenzimol, bisBenzimide H 33342, HOE 33342, HO342. For research use only. ... Concentration (start) x Volume (start) = Concentration (final) x Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output) C1. V1. C2. V2 how to add glassfish server in intellij